PDF DatasheetMouse MKK4 / MEK4 / MAP2K4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MKK4 / MEK4 / MAP2K4 based upon sequence from Mouse
Host: QP6337 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) MKK4 / MEK4 / MAP2K4, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6337.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Asp39
Amino Acid Sequence: AAPSPSGGGG SGGGGGTPGP IGPPASGHPA VSSMQGKRKA LKLNFANPPV KSTARFTLNP NTTGVQNPHI ERLRTHSIES SGKLKISPEQ HWDFTAEDLK DLGEIGRGAY GSVNKMVHKP SGQIMAVKRI RSTVDEKEQK QLLMDLDVVM RSSDCPYIVQ FYGALFREGD CWICMELMST SFDKFYKYVY SVLDDVIPEE ILGKITLATV KALNHLKENL KIIHRDIKPS NILLDRSGNI KLCDFGISGQ LVDSIAKTRD AGCRPYMAPE RIDPSASRQG YDVRSDVWSL GITLYELATG RFPYPKWNSV FDQLTQVVKG DPPQLSNSEE REFSPSFINF VNLCLTKDES KRPKYKELLK HPFILMYEER TVEVACYVCK ILDQMPATPS SPMYVD
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Mouse MKK4 / MEK4 / MAP2K4 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse MKK4 / MEK4 / MAP2K4 Protein General Information | |
|---|---|
| Alternate Names | |
| MKK4, MEK4, MAP2K4, JNKK1, MEK4, PRKMK4, Serk1, MKK4, Sek1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Map2k4 |
| Entrez Gene ID | 26398 |
| Ensemble Gene ID | ENSMUSG00000033352 |
| RefSeq Protein Accession(s) | NP_033183.1 |
| RefSeq mRNA Accession(s) | NM_009157.5 |
| UniProt ID(s) | P47809 |
| UniGene ID(s) | Mm.412922 |
| COSMIC ID Link(s) | Map2k4 |
| KEGG Gene ID(s) | mmu:26398 |
| General Description of Recombinant Mouse MKK4 / MEK4 / MAP2K4 Protein. | |
| Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 ses to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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