PDF DatasheetHuman MKK6 / MEK6 / MAP2K6 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MKK6 / MEK6 / MAP2K6 based upon sequence from Human
Host: QP6338 protein expressed in Baculovirus, E.coli..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) MKK6 / MEK6 / MAP2K6, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6338.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Asp334
Amino Acid Sequence: MSQSKGKKRN PGLKIPKEAF EQPQTSSTPP RDLDSKACIS IGNQNFEVKA DDLEPIMELG RGAYGVVEKM RHVPSGQIMA VKRIRATVNS QEQKRLLMDL DISMRTVDCP FTVTFYGALF REGDVWICME LMDTSLDKFY KQVIDKGQTI PEDILGKIAV SIVKALEHLH SKLSVIHRDV KPSNVLINAL GQVKMCDFGI SGYLVDSVAK TIDAGCKPYM APERINPELN QKGYSVKSDI WSLGITMIEL AILRFPYDSW GTPFQQLKQV VEEPSPQLPA DKFSAEFVDF TSQCLKKNSK ERPTYPELMQ HPFFTLHESK GTDVASFVKL ILGD
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human MKK6 / MEK6 / MAP2K6 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human MKK6 / MEK6 / MAP2K6 Protein General Information | |
|---|---|
| Alternate Names | |
| MKK6, MEK6, MAP2K6, SAPKK3, MKK6, SAPKK-3, MAPKK6, MEK6, PRKMK6 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MAP2K6 |
| Entrez Gene ID | 5608 |
| Ensemble Gene ID | ENSG00000108984 |
| RefSeq Protein Accession(s) | NP_002749.2 |
| RefSeq mRNA Accession(s) | NM_002758.3 |
| UniProt ID(s) | P52564 |
| UniGene ID(s) | Hs.463978 |
| HGNC ID(s) | HGNC:6846 |
| COSMIC ID Link(s) | MAP2K6 |
| KEGG Gene ID(s) | hsa:5608 |
| PharmGKB ID(s) | PA30591 |
| General Description of Recombinant Human MKK6 / MEK6 / MAP2K6 Protein. | |
| Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.