PDF DatasheetHuman JNK2 / MAPK9 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant JNK2 / MAPK9 based upon sequence from Human
Host: QP6342 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) JNK2 / MAPK9, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6342.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Arg424
Amino Acid Sequence: MSDSKCDSQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI NVAVKKLSRP FQNQTHAKRA YRELVLLKCV NHKNIISLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIH MELDHERMSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTACTNF MMTPYVVTRY YRAPEVILGM GYKENVDIWS VGCIMGELVK GCVIFQGTDH IDQWNKVIEQ LGTPSAEFMK KLQPTVRNYV ENRPKYPGIK FEELFPDWIF PSESERDKIK TSQARDLLSK MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIE EWKELIYKEV MDWEERSKNG VVKDQPSDAA VSSNATPSQS SSINDISSMS TEQTLASDTD SSLDASTGPL EGCR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human JNK2 / MAPK9 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human JNK2 / MAPK9 Protein General Information | |
|---|---|
| Alternate Names | |
| JNK2, MAPK9, JNK2B, JNK-55, PRKM9, SAPK, p54a, p54aSAPK, JNK2, JNK2A, JNK2BETA, SAPK1a, JNK2ALPHA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MAPK9 |
| Entrez Gene ID | 5601 |
| Ensemble Gene ID | ENSG00000050748 |
| RefSeq Protein Accession(s) | NP_001128516.1 |
| RefSeq mRNA Accession(s) | NM_001135044.1, NM_001308244.1, NM_002752.4, NM_139068.2, NM_139069.2, NM_139070.2, XM_006714891.2, XM_017009638.1, XM_017009641.1 |
| UniProt ID(s) | P45984 |
| UniGene ID(s) | Hs.484371 |
| HGNC ID(s) | HGNC:6886 |
| COSMIC ID Link(s) | MAPK9 |
| KEGG Gene ID(s) | hsa:5601 |
| PharmGKB ID(s) | PA30630 |
| General Description of Recombinant Human JNK2 / MAPK9 Protein. | |
| Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular domain stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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