PDF DatasheetMouse E3 ubiquitin-protein ligase Mdm2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant E3 ubiquitin-protein ligase Mdm2 based upon sequence from Mouse
Host: QP6353 protein expressed in E.coli, Yeast..
Tag: His
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) E3 ubiquitin-protein ligase Mdm2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6353.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Asn489
Amino Acid Sequence: MCNTNMSVST EGAASTSQIP ASEQETLVRP KPLLLKLLKS VGAQNDTYTM KEIIFYIGQY IMTKRLYDEK QQHIVYCSND LLGDVFGVPS FSVKEHRKIY AMIYRNLVAV SQQDSGTSLS ESRRQPEGGS DLKDPLQAPP EEKPSSSDLI SRLSTSSRRR SISETEENTD ELPGERHRKR RRSLSFDPSL GLCELREMCS GGSSSSSSSS SESTETPSHQ DLDDGVSEHS GDCLDQDSVS DQFSVEFEVE SLDSEDYSLS DEGHELSDED DEVYRVTVYQ TGESDTDSFE GDPEISLADY WKCTSCNEMN PPLPSHCKRC WTLRENWLPD DKGKDKVEIS EKAKLENSAQ AEEGLDVPDG KKLTENDAKE PCAEEDSEEK AEQTPLSQES DDYSQPSTSS SIVYSSQESV KELKEETQDK DESVESSFSL NAIEPCVICQ GRPKNGCIVH GKTGHLMSCF TCAKKLKKRN KPCPVCRQPI QMIVLTYFN
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Mouse E3 ubiquitin-protein ligase Mdm2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse E3 ubiquitin-protein ligase Mdm2 Protein General Information | |
|---|---|
| Alternate Names | |
| Mdm-2, AA415488, 1700007J15Rik | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Mdm2 |
| Entrez Gene ID | 17246 |
| Ensemble Gene ID | ENSMUSG00000020184 |
| RefSeq Protein Accession(s) | NP_001275515.1 |
| RefSeq mRNA Accession(s) | NM_001288586.2, NM_010786.4 |
| UniProt ID(s) | P23804 |
| UniGene ID(s) | Mm.22670 |
| COSMIC ID Link(s) | Mdm2 |
| KEGG Gene ID(s) | mmu:17246 |
| General Description of Recombinant Mouse E3 ubiquitin-protein ligase Mdm2 Protein. | |
| E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes th to proteasomal degradation . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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