PDF DatasheetHuman NDST1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant NDST1 based upon sequence from Human
Isoform: Isoform 2
Host: QP6409 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) NDST1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6409.
Bioactivity Data: Untested
Full Length? Full Length of Isoform 2
Expression Region: Gly40 – Ala556
Amino Acid Sequence: GWKRGLEPSA DAPEPDCGDP PPVAPSRLLP LKPVQAATPS RTDPLVLVFV ESLYSQLGQE VVAILESSRF KYRTEIAPGK GDMPTLTDKG RGRFALIIYE NILKYVNLDA WNRELLDKYC VAYGVGIIGF FKANENSLLS AQLKGFPLFL HSNLGLKDCS INPKSPLLYV TRPSEVEKGV LPGEDWTVFQ SNHSTYEPVL LAKTRSSESI PHLGADAGLH AALHATVVQD LGLHDGIQRV LFGNNLNFWL HKLVFVDAVA FLTGKRLSLP LDRYILVDID DIFVGKEGTR MKVEDVKALF DTQNELRAHI PNFTFNLGYS GKFFHTGTNA EDAGDDLLLS YVKEFWWFPH MWSHMQPHLF HNQSVLAEQM ALNKKFAVEH GIPTDMGYAV APHHSGVYPV HVQLYEAWKQ VWSIRVTSTE EYPHLKPARY RRGFIHNGIM VLPRQTCGLF THTIFYNEYP GGSSELDKII NGGELFLTVL LNPVSAPQPM AAGEKGLLHS LSAADTGFLE PGKGGEA
Purity: Greater than 83.3% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human NDST1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human NDST1 Protein General Information | |
|---|---|
| Alternate Names | |
| HSST, NST1, MRT46, Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase | |
| Molecular Weight | |
| Isoform 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NDST1 |
| Entrez Gene ID | 3340 |
| Ensemble Gene ID | ENSG00000070614 |
| RefSeq Protein Accession(s) | NP_001287992.1 |
| RefSeq mRNA Accession(s) | NM_001301063.1, NM_001543.4, XM_006714783.1, XM_017009428.1, XM_017009429.1, XM_017009430.1, XM_017009431.1 |
| UniProt ID(s) | P52848 |
| UniGene ID(s) | Hs.222055 |
| HGNC ID(s) | HGNC:7680 |
| COSMIC ID Link(s) | NDST1 |
| KEGG Gene ID(s) | hsa:3340 |
| PharmGKB ID(s) | PA31486 |
| General Description of Recombinant Human NDST1 Protein. | |
| Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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