PDF DatasheetHuman Nucleoside diphosphate kinase, mitochondrial Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Nucleoside diphosphate kinase, mitochondrial based upon sequence from Human
Host: QP6425 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Nucleoside diphosphate kinase, mitochondrial, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6425.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ser1 – Ala187
Amino Acid Sequence: SWTRERTLVA VKPDGVQRRL VGDVIQRFER RGFTLVGMKM LQAPESVLAE HYQDLRRKPF YPALIRYMSS GPVVAMVWEG YNVVRASRAM IGHTDSAEAA PGTIRGDFSV HISRNVIHAS DSVEGAQREI QLWFQSSELV SWADGGQHSS IHPA
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Nucleoside diphosphate kinase, mitochondrial Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Nucleoside diphosphate kinase, mitochondrial Protein General Information | |
|---|---|
| Alternate Names | |
| NDPK-D, nm23-H4, NM23H4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NME4 |
| Entrez Gene ID | 4833 |
| Ensemble Gene ID | ENSG00000103202 |
| RefSeq Protein Accession(s) | NP_001273362.1 |
| RefSeq mRNA Accession(s) | NM_001286433.1, NM_001286435.1, NM_001286436.1, NM_001286438.1, NM_001286439.1, NM_001286440.1, NM_005009.2 |
| UniProt ID(s) | O00746 |
| UniGene ID(s) | Hs.9235 |
| HGNC ID(s) | HGNC:7852 |
| COSMIC ID Link(s) | NME4 |
| KEGG Gene ID(s) | hsa:4833 |
| PharmGKB ID(s) | PA31657 |
| General Description of Recombinant Human Nucleoside diphosphate kinase, mitochondrial Protein. | |
| Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis. Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity. Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration. Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria. In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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