PDF DatasheetHuman OGT1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant OGT1 based upon sequence from Human
Host: QP6450 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) OGT1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6450.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Met606 – Gln1022
Amino Acid Sequence: MAEANHFIDL SQIPCNGKAA DRIHQDGIHI LVNMNGYTKG ARNELFALRP APIQAMWLGY PGTSGALFMD YIITDQETSP AEVAEQYSEK LAYMPHTFFI GDHANMFPHL KKKAVIDFKS NGHIYDNRIV LNGIDLKAFL DSLPDVKIVK MKCPDGGDNA DSSNTALNMP VIPMNTIAEA VIEMINRGQI QITINGFSIS NGLATTQINN KAATGEEVPR TIIVTTRSQY GLPEDAIVYC NFNQLYKIDP STLQMWANIL KRVPNSVLWL LRFPAVGEPN IQQYAQNMGL PQNRIIFSPV APKEEHVRRG QLADVCLDTP LCNGHTTGMD VLWAGTPMVT MPGETLASRV AASQLTCLGC LELIAKNRQE YEDIAVKLGT DLEYLKKVRG KVWKQRISSP LFNTKQYTME LERLYLQ
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human OGT1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human OGT1 Protein General Information | |
|---|---|
| Alternate Names | |
| O-GLCNAC, HINCUT-1, HRNT1, UDP-N-acetylglucosamine–peptide N-acetylglucosaminyltransferase 110 kDa subun | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | OGT |
| Entrez Gene ID | 8473 |
| Ensemble Gene ID | ENSG00000147162 |
| RefSeq Protein Accession(s) | NP_858058.1 |
| RefSeq mRNA Accession(s) | NM_181672.2, NM_181673.2, XM_017029907.1, XM_017029908.1 |
| UniProt ID(s) | O15294 |
| UniGene ID(s) | Hs.405410 |
| HGNC ID(s) | HGNC:8127 |
| COSMIC ID Link(s) | OGT |
| KEGG Gene ID(s) | hsa:8473 |
| PharmGKB ID(s) | PA31914 |
| General Description of Recombinant Human OGT1 Protein. | |
| Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in Cytoplasmic domain and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the ‘Thr-308’ phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity . Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of ‘Ser-112’ of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) . As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues . O-GlcNAcylation of ‘Ser-75’ of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex . Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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