PDF DatasheetHuman Serine / threonine-protein kinase PAK 4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Serine / threonine-protein kinase PAK 4 based upon sequence from Human
Isoform: Isoform 2
Host: QP6464 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Serine/threonine-protein kinase PAK 4, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6464.
Bioactivity Data: Untested
Full Length? Full Length of Isoform 2
Expression Region: Met1 – Arg426
Amino Acid Sequence: MFGKRKKRVE ISAPSNFEHR VHTGFDQHEQ KFTGLPRQWQ SLIEESARRP KPLVDPACIT SIQPGAPKTI VRGSKGAKDG ALTLLLDEFE NMSVTRSNSL RRDSPPPPAR ARQENGMPEK PPGPRSPQRE PQRVSHEQFR AALQLVVDPG DPRSYLDNFI KIGEGSTGIV CIATVRSSGK LVAVKKMDLR KQQRRELLFN EVVIMRDYQH ENVVEMYNSY LVGDELWVVM EFLEGGALTD IVTHTRMNEE QIAAVCLAVL QALSVLHAQG VIHRDIKSDS ILLTHDGRVK LSDFGFCAQV SKEVPRRKSL VGTPYWMAPE LISRLPYGPE VDIWSLGIMV IEMVDGEPPY FNEPPLKAMK MIRDNLPPRL KNLHKVSPSL KGFLDRLLVR DPAQRATAAE LLKHPFLAKA GPPASIVPLM RQNRTR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Serine / threonine-protein kinase PAK 4 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Serine / threonine-protein kinase PAK 4 Protein General Information | |
|---|---|
| Molecular Weight | |
| Isoform 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PAK4 |
| Entrez Gene ID | 10298 |
| Ensemble Gene ID | ENSG00000130669 |
| RefSeq Protein Accession(s) | NP_001014831.1 |
| RefSeq mRNA Accession(s) | NM_001014831.2, NM_001014832.1, NM_001014834.2, NM_001014835.1, NM_005884.3, XM_011526316.1, XM_011526317.2, XM_011526318.2, XM_011526319.2, XM_011526320.1 |
| UniProt ID(s) | O96013 |
| UniGene ID(s) | Hs.20447 |
| HGNC ID(s) | HGNC:16059 |
| COSMIC ID Link(s) | PAK4 |
| KEGG Gene ID(s) | hsa:10298 |
| PharmGKB ID(s) | PA32920 |
| General Description of Recombinant Human Serine / threonine-protein kinase PAK 4 Protein. | |
| Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assbly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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