PDF DatasheetHuman Poly(A)-specific ribonuclease PARN Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Poly(A)-specific ribonuclease PARN based upon sequence from Human
Host: QP6467 protein expressed in E.coli, Yeast..
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Poly(A)-specific ribonuclease PARN, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6467.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Trp639
Amino Acid Sequence: MEIIRSNFKS NLHKVYQAIE EADFFAIDGE FSGISDGPSV SALTNGFDTP EERYQKLKKH SMDFLLFQFG LCTFKYDYTD SKYITKSFNF YVFPKPFNRS SPDVKFVCQS SSIDFLASQG FDFNKVFRNG IPYLNQEEER QLREQYDEKR SQANGAGALS YVSPNTSKCP VTIPEDQKKF IDQVVEKIED LLQSEENKNL DLEPCTGFQR KLIYQTLSWK YPKGIHVETL ETEKKERYIV ISKVDEEERK RREQQKHAKE QEELNDAVGF SRVIHAIANS GKLVIGHNML LDVMHTVHQF YCPLPADLSE FKEMTTCVFP RLLDTKLMAS TQPFKDIINN TSLAELEKRL KETPFNPPKV ESAEGFPSYD TASEQLHEAG YDAYITGLCF ISMANYLGSF LSPPKIHVSA RSKLIEPFFN KLFLMRVMDI PYLNLEGPDL QPKRDHVLHV TFPKEWKTSD LYQLFSAFGN IQISWIDDTS AFVSLSQPEQ VKIAVNTSKY AESYRIQTYA EYMGRKQEEK QIKRKWTEDS WKEADSKRLN PQCIPYTLQN HYYRNNSFTA PSTVGKRNLS PSQEEAGLED GVSGEISDTE LEQTDSCAEP LSEGRKKAKK LKRMKKELSP AGSISKNSPA TLFEVPDTW
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Poly(A)-specific ribonuclease PARN Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Poly(A)-specific ribonuclease PARN Protein General Information | |
|---|---|
| Alternate Names | |
| PFBMFT4, DAN, DKCB6 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PARN |
| Entrez Gene ID | 5073 |
| Ensemble Gene ID | ENSG00000140694 |
| RefSeq Protein Accession(s) | NP_001127949.1 |
| RefSeq mRNA Accession(s) | NM_001134477.2, NM_001242992.1, NM_002582.3 |
| UniProt ID(s) | O95453 |
| UniGene ID(s) | Hs.253197 |
| HGNC ID(s) | HGNC:8609 |
| COSMIC ID Link(s) | PARN |
| KEGG Gene ID(s) | hsa:5073 |
| PharmGKB ID(s) | PA29072 |
| General Description of Recombinant Human Poly(A)-specific ribonuclease PARN Protein. | |
| 3′-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early bryonic development. Interacts with both the 3′-end poly(A) tail and the 5′-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain prature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elents (AREs) in their 3′-UTR, possibly via its interaction with KHSRP. Probably mediates the roval of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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