![SDS-PAGE separation of QP6468 followed by commassie total protein stain results in a primary band consistent with reported data for Poly [ADP-ribose] polymerase 1. These data demonstrate Greater than 90% as determined by SDS-PAGE.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2018/01/QP6468-Human-Poly-ADP-ribose-polymerase-1-1.jpg "SDS-PAGE separation of QP6468 followed by commassie total protein stain results in a primary band consistent with reported data for Poly [ADP-ribose] polymerase 1. These data demonstrate Greater than 90% as determined by SDS-PAGE.")
PDF DatasheetHuman Poly [ADP-ribose] polymerase 1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Poly [ADP-ribose] polymerase 1 based upon sequence from Human
Host: QP6468 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Poly [ADP-ribose] polymerase 1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6468.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Ala2 – Lys331
Amino Acid Sequence: AESSDKLYRV EYAKSGRASC KKCSESIPKD SLRMAIMVQS PMFDGKVPHW YHFSCFWKVG HSIRHPDVEV DGFSELRWDD QQKVKKTAEA GGVTGKGQDG IGSKAEKTLG DFAAEYAKSN RSTCKGCMEK IEKGQVRLSK KMVDPEKPQL GMIDRWYHPG CFVKNREELG FRPEYSASQL KGFSLLATED KEALKKQLPG VKSEGKRKGD EVDGVDEVAK KKSKKEKDKD SKLEKALKAQ NDLIWNIKDE LKKVCSTNDL KELLIFNKQQ VPSGESAILD RVADGMVFGA LLPCEECSGQ LVFKSDAYYC TGDVTAWTKC MVKTQTPNRK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Poly [ADP-ribose] polymerase 1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Poly Protein polymerase 1 Protein General Information | |
|---|---|
| Alternate Names | |
| PARP, PARP-1, ADPRT1, PPOL, pADPRT-1, ADPRT, ADPRT 1, ARTD1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PARP11 |
| Entrez Gene ID | 142 |
| Ensemble Gene ID | ENSG00000143799 |
| RefSeq Protein Accession(s) | NP_001609.2 |
| RefSeq mRNA Accession(s) | NM_001618.3 |
| UniProt ID(s) | P09874 |
| UniGene ID(s) | Hs.177766 |
| HGNC ID(s) | HGNC:270 |
| COSMIC ID Link(s) | PARP11 |
| KEGG Gene ID(s) | hsa:142 |
| PharmGKB ID(s) | PA32 |
| General Description of Recombinant Human Poly Protein polymerase 1 Protein. | |
| Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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