PDF DatasheetHuman DNA-directed RNA polymerase III subunit RPC1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DNA-directed RNA polymerase III subunit RPC1 based upon sequence from Human
Host: QP6511 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) DNA-directed RNA polymerase III subunit RPC1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6511.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Phe392 – Cys632
Amino Acid Sequence: FPEKVNKANI NFLRKLVQNG PEVHPGANFI QQRHTQMKRF LKYGNREKMA QELKYGDIVE RHLIDGDVVL FNRQPSLHKL SIMAHLARVK PHRTFRFNEC VCTPYNADFD GDEMNLHLPQ TEEAKAEALV LMGTKANLVT PRNGEPLIAA IQDFLTGAYL LTLKDTFFDR AKACQIIASI LVGKDEKIKV RLPPPTILKP VTLWTGKQIF SVILRPSDDN PVRANLRTKG KQYCGKGEDL C
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human DNA-directed RNA polymerase III subunit RPC1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human DNA-directed RNA polymerase III subunit RPC1 Protein General Information | |
|---|---|
| Alternate Names | |
| RPC1, hRPC155, ADDH, HLD7, RPC155 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | POLR3A |
| Entrez Gene ID | 11128 |
| Ensemble Gene ID | ENSG00000148606 |
| RefSeq Protein Accession(s) | NP_008986.2 |
| RefSeq mRNA Accession(s) | NM_007055.3 |
| UniProt ID(s) | O14802 |
| UniGene ID(s) | Hs.436896 |
| HGNC ID(s) | HGNC:30074 |
| COSMIC ID Link(s) | POLR3A |
| KEGG Gene ID(s) | hsa:11128 |
| PharmGKB ID(s) | PA134900426 |
| General Description of Recombinant Human DNA-directed RNA polymerase III subunit RPC1 Protein. | |
| DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA tplate strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix anates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition . Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as tplate for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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