PDF DatasheetMouse PRKDC Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PRKDC based upon sequence from Mouse
Host: QP6529 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) PRKDC, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6529.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Glu3747 – Ser4015
Amino Acid Sequence: EYPFLVKGGE DLRQDQRIEQ IFEVMNAILS QDAACSQRNM QLRTYRVVPM TSRLGLIEWI ENTMTLKDLL LSNMSQEEKV ANNSDPKAPI RDYKDWLMKV SGKSDAGAYV LMYSRANRTE TVVAFRRRES QVPPDLLKRA FVKMSTSPEA FLALRSHFAS SHALLCISHW LLGIGDRHLN NFMVAMETGS VIGIDFGHAF GSATQFLPVP ELMPFRLTRQ FVSLMLPMKE TGLMCTVMVH ALRAFRSCAG LLTDTMEIFV KEPSFDWKS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Mouse PRKDC Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse PRKDC Protein General Information | |
|---|---|
| Alternate Names | |
| AI326420, scid, dxnph, p460, slip, DNAPK, DNPK1, XRCC7, DNA-PKcs, DNAPDcs, HYRC1, AU019811, DOXNPH, DNA-dependent protein kinase catalytic subun | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PRKDC |
| Entrez Gene ID | 19090 |
| Ensemble Gene ID | ENSMUSG00000022672 |
| RefSeq Protein Accession(s) | NP_035289.2 |
| RefSeq mRNA Accession(s) | NM_011159.2 |
| UniProt ID(s) | P97313 |
| UniGene ID(s) | Mm.71 |
| KEGG Gene ID(s) | mmu:19090 |
| General Description of Recombinant Mouse PRKDC Protein. | |
| Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artis (DCLRE1C). The assbly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates ‘Ser-139’ of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, C1D, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 ‘Ser-588’ and increasing CRY1 protein stability, most likely through an indirect machanism. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.