PDF DatasheetHuman PSMD10 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PSMD10 based upon sequence from Human
Host: QP6546 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) PSMD10, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6546.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Gly226
Amino Acid Sequence: MEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human PSMD10 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human PSMD10 Protein General Information | |
|---|---|
| Alternate Names | |
| dJ889N15.2, p28(GANK), p28, 26S proteasome non-ATPase regulatory subunit | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PSMD10 |
| Entrez Gene ID | 5716 |
| Ensemble Gene ID | ENSG00000101843 |
| RefSeq Protein Accession(s) | NP_002805.1 |
| RefSeq mRNA Accession(s) | NM_002814.3, NM_170750.2 |
| UniProt ID(s) | O75832 |
| UniGene ID(s) | Hs.522752 |
| HGNC ID(s) | HGNC:9555 |
| COSMIC ID Link(s) | PSMD10 |
| KEGG Gene ID(s) | hsa:5716 |
| PharmGKB ID(s) | PA33900 |
| General Description of Recombinant Human PSMD10 Protein. | |
| Acts as a chaperone during the assbly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assbly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assbles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.