PDF DatasheetHuman RPA1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant RPA1 based upon sequence from Human
Host: QP6618 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) RPA1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6618.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Val2 – Met616
Amino Acid Sequence: VGQLSEGAIA AIMQKGDTNI KPILQVINIR PITTGNSPPR YRLLMSDGLN TLSSFMLATQ LNPLVEEEQL SSNCVCQIHR FIVNTLKDGR RVVILMELEV LKSAEAVGVK IGNPVPYNEG LGQPQVAPPA PAASPAASSR PQPQNGSSGM GSTVSKAYGA SKTFGKAAGP SLSHTSGGTQ SKVVPIASLT PYQSKWTICA RVTNKSQIRT WSNSRGEGKL FSLELVDESG EIRATAFNEQ VDKFFPLIEV NKVYYFSKGT LKIANKQFTA VKNDYEMTFN NETSVMPCED DHHLPTVQFD FTGIDDLENK SKDSLVDIIG ICKSYEDATK ITVRSNNREV AKRNIYLMDT SGKVVTATLW GEDADKFDGS RQPVLAIKGA RVSDFGGRSL SVLSSSTIIA NPDIPEAYKL RGWFDAEGQA LDGVSISDLK SGGVGGSNTN WKTLYEVKSE NLGQGDKPDY FSSVATVVYL RKENCMYQAC PTQDCNKKVI DQQNGLYRCE KCDTEFPNFK YRMILSVNIA DFQENQWVTC FQESAEAILG QNAAYLGELK DKNEQAFEEV FQNANFRSFI FRVRVKVETY NDESRIKATV MDVKPVDYRE YGRRLVMSIR RSALM
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human RPA1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human RPA1 Protein General Information | |
|---|---|
| Alternate Names | |
| REPA1, RF-A, RP-A, MST075, HSSB, RPA70, Replication protein A 70 kDa DNA-binding subun | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | RPA1 |
| Entrez Gene ID | 6117 |
| Ensemble Gene ID | ENSG00000132383 |
| RefSeq Protein Accession(s) | NP_002936.1 |
| RefSeq mRNA Accession(s) | NM_002945.3 |
| UniProt ID(s) | P27694 |
| UniGene ID(s) | Hs.461925 |
| HGNC ID(s) | HGNC:10289 |
| COSMIC ID Link(s) | RPA1 |
| KEGG Gene ID(s) | hsa:6117 |
| PharmGKB ID(s) | PA34651 |
| General Description of Recombinant Human RPA1 Protein. | |
| As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage . In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response . It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage . Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair . Plays also a role in base excision repair (BER) probably through interaction with UNG . Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance . As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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