PDF DatasheetMouse 40S ribosomal protein S3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant 40S ribosomal protein S3 based upon sequence from Mouse
Host: QP6627 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) 40S ribosomal protein S3, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6627.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Ala243
Amino Acid Sequence: AVQISKKRKF VADGIFKAEL NEFLTRELAE DGYSGVEVRV TPTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG VLRFIMESGA KGCEVVVSGK LRGQRAKSMK FVDGLMIHSG DPVNYYVDTA VRHVLLRQGV LGIKVKIMLP WDPTGKIGPK KPLPDHVSIV EPKDEILPTT PISEQKGGKP EPPAMPQPVP TA
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Mouse 40S ribosomal protein S3 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse 40S ribosomal protein S3 Protein General Information | |
|---|---|
| Alternate Names | |
| S3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | RPS3 |
| Entrez Gene ID | 6188 |
| Ensemble Gene ID | ENSG00000149273 |
| RefSeq Protein Accession(s) | NP_000996.2 |
| RefSeq mRNA Accession(s) | NM_001005.4, NM_001256802.1, NM_001260506.1, NM_001260507.1 |
| UniProt ID(s) | P23396 |
| UniGene ID(s) | Hs.546286 |
| HGNC ID(s) | HGNC:10420 |
| COSMIC ID Link(s) | RPS3 |
| KEGG Gene ID(s) | hsa:6188 |
| PharmGKB ID(s) | PA34829 |
| General Description of Recombinant Mouse 40S ribosomal protein S3 Protein. | |
| Involved in translation as a component of the 40S small ribosomal subunit (PubMed:8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571). Enhances the uracil excision activity of UNG1 (PubMed:18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed:17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed:18045535). Represses its own translation by binding to its cognate mRNA (PubMed:20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed:19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed:23131551). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed:20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed:22510408). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.