PDF DatasheetHuman Serine / arginine-rich splicing factor 1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Serine / arginine-rich splicing factor 1 based upon sequence from Human
Host: QP6679 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Serine/arginine-rich splicing factor 1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6679.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ser2 – Thr248
Amino Acid Sequence: SGGGVIRGPA GNNDCRIYVG NLPPDIRTKD IEDVFYKYGA IRDIDLKNRR GGPPFAFVEF EDPRDAEDAV YGRDGYDYDG YRLRVEFPRS GRGTGRGGGG GGGGGAPRGR YGPPSRRSEN RVVVSGLPPS GSWQDLKDHM REAGDVCYAD VYRDGTGVVE FVRKEDMTYA VRKLDNTKFR SHEGETAYIR VKVDGPRSPS YGRSRSRSRS RSRSRSRSNS RSRSYSPRRS RGSPRYSPRH SRSRSRT
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Serine / arginine-rich splicing factor 1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Serine / arginine-rich splicing factor 1 Protein General Information | |
|---|---|
| Alternate Names | |
| SFRS1, SRp30a, ASF, SF2, SF2p33 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SRSF1 |
| Entrez Gene ID | 6426 |
| Ensemble Gene ID | ENSG00000136450 |
| RefSeq Protein Accession(s) | NP_001071634.1 |
| RefSeq mRNA Accession(s) | NM_001078166.1, NM_006924.4 |
| UniProt ID(s) | Q07955 |
| UniGene ID(s) | Hs.68714 |
| HGNC ID(s) | HGNC:10780 |
| COSMIC ID Link(s) | SRSF1 |
| KEGG Gene ID(s) | hsa:6426 |
| PharmGKB ID(s) | PA35696 |
| General Description of Recombinant Human Serine / arginine-rich splicing factor 1 Protein. | |
| Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5′- and 3′-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5′-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5′-RGAAGAAC-3′ (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5′-CGAGGCG-3′ motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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