PDF DatasheetHuman S-phase kinase-associated protein 1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant S-phase kinase-associated protein 1 based upon sequence from Human
Host: QP6689 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) S-phase kinase-associated protein 1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6689.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Pro2 – Lys163
Amino Acid Sequence: PSIKLQSSDG EIFEVDVEIA KQSVTIKTML EDLGMDDEGD DDPVPLPNVN AAILKKVIQW CTHHKDDPPP PEDDENKEKR TDDIPVWDQE FLKVDQGTLF ELILAANYLD IKGLLDVTCK TVANMIKGKT PEEIRKTFNI KNDFTEEEEA QVRKENQWCE EK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human S-phase kinase-associated protein 1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human S-phase kinase-associated protein 1 Protein General Information | |
|---|---|
| Alternate Names | |
| OCP2, EMC19, OCP-II, p19A, TCEB1L, SKP1A | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SKP1 |
| Entrez Gene ID | 6500 |
| Ensemble Gene ID | ENSG00000113558 |
| RefSeq Protein Accession(s) | NP_008861.2 |
| RefSeq mRNA Accession(s) | NM_006930.3, NM_170679.2 |
| UniProt ID(s) | P63208 |
| UniGene ID(s) | Hs.171626 |
| HGNC ID(s) | HGNC:10899 |
| COSMIC ID Link(s) | SKP1 |
| KEGG Gene ID(s) | hsa:6500 |
| PharmGKB ID(s) | PA162403424 |
| General Description of Recombinant Human S-phase kinase-associated protein 1 Protein. | |
| Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not se to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at ‘Lys-21’ and ‘Lys-22’; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) direct ubiquitination of TTI1 and TELO2. SCF(FBXO10) direct ubiquitination of BCL2. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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