PDF DatasheetHuman Transcription activator BRG1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Transcription activator BRG1 based upon sequence from Human
Host: QP6699 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Transcription activator BRG1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6699.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Ile808 – Leu1092
Amino Acid Sequence: IIVPLSTLSN WAYEFDKWAP SVVKVSYKGS PAARRAFVPQ LRSGKFNVLL TTYEYIIKDK HILAKIRWKY MIVDEGHRMK NHHCKLTQVL NTHYVAPRRL LLTGTPLQNK LPELWALLNF LLPTIFKSCS TFEQWFNAPF AMTGEKVDLN EEETILIIRR LHKVLRPFLL RRLKKEVEAQ LPEKVEYVIK CDMSALQRVL YRHMQAKGVL LTDGSEKDKK GKGGTKTLMN TIMQLRKICN HPYMFQHIEE SFSEHLGFTG GIVQGLDLYR ASGKFELLDR ILPKL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Transcription activator BRG1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Transcription activator BRG1 Protein General Information | |
|---|---|
| Alternate Names | |
| BAF190, RTPS2, SNF2, hSNF2b, CSS4, SWI2, BAF190A, MRD16, SNF2LB, BRG1, SNF2L4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SMARCA4 |
| Entrez Gene ID | 6597 |
| Ensemble Gene ID | ENSG00000127616 |
| RefSeq Protein Accession(s) | NP_001122316.1 |
| RefSeq mRNA Accession(s) | NM_001128844.1, NM_001128845.1, NM_001128846.1, NM_001128847.1, NM_001128848.1, NM_003072.3, XM_017027165.1, XM_017027166.1, XM_017027167.1, XM_017027168.1 |
| UniProt ID(s) | P51532 |
| UniGene ID(s) | Hs.327527 |
| HGNC ID(s) | HGNC:11100 |
| COSMIC ID Link(s) | SMARCA4 |
| KEGG Gene ID(s) | hsa:6597 |
| PharmGKB ID(s) | PA35950 |
| General Description of Recombinant Human Transcription activator BRG1 Protein. | |
| Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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