PDF DatasheetHuman Transcription elongation factor SPT4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Transcription elongation factor SPT4 based upon sequence from Human
Host: QP6753 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Transcription elongation factor SPT4, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6753.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala1 – Thr117
Amino Acid Sequence: ALETVPKDLR HLRACLLCSL VKTIDQFEYD GCDNCDAYLQ MKGNREMVYD CTSSSFDGII AMMSPEDSWV SKWQRVSNFK PGVYAVSVTG RLPQGIVREL KSRGVAYKSR DTAIKT
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Transcription elongation factor SPT4 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Transcription elongation factor SPT4 Protein General Information | |
|---|---|
| Alternate Names | |
| SPT4H, SUPT4H, Supt4a, SPT4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SUPT4H1 |
| Entrez Gene ID | 6827 |
| Ensemble Gene ID | ENSG00000213246 |
| RefSeq Protein Accession(s) | NP_003159.1 |
| RefSeq mRNA Accession(s) | NM_003168.2 |
| UniProt ID(s) | P63272 |
| UniGene ID(s) | Hs.439481 |
| HGNC ID(s) | HGNC:11467 |
| COSMIC ID Link(s) | SUPT4H1 |
| KEGG Gene ID(s) | hsa:6827 |
| PharmGKB ID(s) | PA36253 |
| General Description of Recombinant Human Transcription elongation factor SPT4 Protein. | |
| Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3′ terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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