PDF DatasheetMouse Ubiquitin-protein ligase E3A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Ubiquitin-protein ligase E3A based upon sequence from Mouse
Host: QP6865 protein expressed in E.coli, Yeast, Mammal..
Tag: Flag
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) Ubiquitin-protein ligase E3A, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6865.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: His542 – Val870
Amino Acid Sequence: HIIDDALVRL EMIAMENPAD LKKQLYVEFE GEQGVDEGGV SKEFFQLVVE EIFNPDIGMF TYDEATKLFW FNPSSFETEG QFTLIGILGL AIYNNCILDV HFPMVVYRKL MGKKGTFRDL GDSHPVLYQS LKDLLEYEGS VEDDMMITFQ ISQTDLFGNP MMYDLKENGD KIPITNENRK EFVISYSDYI LNKSVEKQFK AFRRGFHMVT NESPLKYLFR PEEIELLICG SRNLDFQALE ETTEYDGGYT RESVVIREFW EIVHSFTDEQ KRLFLLFTTG TDRAPVGGLG KLKMIIAKNG PDTERLPTSH TCFNVLLLPE YSSKEKLNV
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Mouse Ubiquitin-protein ligase E3A Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse Ubiquitin-protein ligase E3A Protein General Information | |
|---|---|
| Alternate Names | |
| A130086L21Rik, mKIAA4216, 4732496B02, 5830462N02Rik, Hpve6a | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ube3a |
| Entrez Gene ID | 22215 |
| Ensemble Gene ID | ENSMUSG00000025326 |
| RefSeq Protein Accession(s) | NP_035798.2 |
| RefSeq mRNA Accession(s) | NM_011668.2, NM_173010.3, XM_006540799.3, XM_011250847.2, XM_017322125.1 |
| UniProt ID(s) | O08759 |
| UniGene ID(s) | Mm.9002 |
| COSMIC ID Link(s) | Ube3a |
| KEGG Gene ID(s) | mmu:22215 |
| General Description of Recombinant Mouse Ubiquitin-protein ligase E3A Protein. | |
| E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo (By similarity). Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990) | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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