PDF DatasheetHuman DNA repair protein complementing XP-C cells Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DNA repair protein complementing XP-C cells based upon sequence from Human
Host: QP6892 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) DNA repair protein complementing XP-C cells, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6892.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Ser496 – Gly734
Amino Acid Sequence: SLPAASSSSS SSKRGKKMCS DGEKAEKRSI AGIDQWLEVF CEQEEKWVCV DCVHGVVGQP LTCYKYATKP MTYVVGIDSD GWVRDVTQRY DPVWMTVTRK CRVDAEWWAE TLRPYQSPFM DREKKEDLEF QAKHMDQPLP TAIGLYKNHP LYALKRHLLK YEAIYPETAA ILGYCRGEAV YSRDCVHTLH SRDTWLKKAR VVRLGEVPYK MVKGFSNRAR KARLAEPQLR EENDLGLFG
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human DNA repair protein complementing XP-C cells Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human DNA repair protein complementing XP-C cells Protein General Information | |
|---|---|
| Alternate Names | |
| XP3, XPCC, p125, RAD4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | XPC |
| Entrez Gene ID | 7508 |
| Ensemble Gene ID | ENSG00000154767 |
| RefSeq Protein Accession(s) | NP_004619.3 |
| RefSeq mRNA Accession(s) | NM_004628.4 |
| UniProt ID(s) | Q01831 |
| UniGene ID(s) | Hs.475538 |
| HGNC ID(s) | HGNC:12816 |
| COSMIC ID Link(s) | XPC |
| KEGG Gene ID(s) | hsa:7508 |
| PharmGKB ID(s) | PA37413 |
| General Description of Recombinant Human DNA repair protein complementing XP-C cells Protein. | |
| Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5′-to-3′ direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5′ and 3′ of a cisplatin lesion with a preference for the 5′ side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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