PDF DatasheetHuman X-ray repair cross-complementing protein 5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant X-ray repair cross-complementing protein 5 based upon sequence from Human
Host: QP6894 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) X-ray repair cross-complementing protein 5, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6894.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Leu251 – Asp455
Amino Acid Sequence: LTIGSNLSIR IAAYKSILQE RVKKTWTVVD AKTLKKEDIQ KETVYCLNDD DETEVLKEDI IQGFRYGSDI VPFSKVDEEQ MKYKSEGKCF SVLGFCKSSQ VQRRFFMGNQ VLKVFAARDD EAAAVALSSL IHALDDLDMV AIVRYAYDKR ANPQVGVAFP HIKHNYECLV YVQLPFMEDL RQYMFSSLKN SKKYAPTEAQ LNAVD
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human X-ray repair cross-complementing protein 5 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human X-ray repair cross-complementing protein 5 Protein General Information | |
|---|---|
| Alternate Names | |
| KARP-1, KU80, NFIV, KARP1, KUB2, Ku86 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | XRCC5 |
| Entrez Gene ID | 7520 |
| Ensemble Gene ID | ENSG00000079246 |
| RefSeq Protein Accession(s) | NP_066964.1 |
| RefSeq mRNA Accession(s) | NM_021141.3 |
| UniProt ID(s) | P13010 |
| UniGene ID(s) | Hs.388739 |
| HGNC ID(s) | HGNC:12833 |
| COSMIC ID Link(s) | XRCC5 |
| KEGG Gene ID(s) | hsa:7520 |
| PharmGKB ID(s) | PA37425 |
| General Description of Recombinant Human X-ray repair cross-complementing protein 5 Protein. | |
| Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3′-5′ direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing th together. The assbly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5′-deoxyribose-5-phosphate lyase (5′-dRP lyase), by catalyzing the beta-elimination of the 5′ deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5′-dRP activity, and allows to ‘clean’ the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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