PDF DatasheetE. coli Glutamate decarboxylase alpha Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Glutamate decarboxylase alpha based upon sequence from: E. coli
Host: QP6947 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Glutamate decarboxylase alpha, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6947.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Thr466
Amino Acid Sequence: MDQKLLTDFR SELLDSRFGA KAISTIAESK RFPLHEMRDD VAFQIINDEL YLDGNARQNL ATFCQTWDDE NVHKLMDLSI NKNWIDKEEY PQSAAIDLRC VNMVADLWHA PAPKNGQAVG TNTIGSSEAC MLGGMAMKWR WRKRMEAAGK PTDKPNLVCG PVQICWHKFA RYWDVELREI PMRPGQLFMD PKRMIEACDE NTIGVVPTFG VTYTGNYEFP QPLHDALDKF QADTGIDIDM HIDAASGGFL APFVAPDIVW DFRLPRVKSI SASGHKFGLA PLGCGWVIWR DEEALPQELV FNVDYLGGQI GTFAINFSRP AGQVIAQYYE FLRLGREGYT KVQNASYQVA AYLADEIAKL GPYEFICTGR PDEGIPAVCF KLKDGEDPGY TLYDLSERLR LRGWQVPAFT LGGEATDIVV MRIMCRRGFE MDFAELLLED YKASLKYLSD HPKLQGIAQQ NSFKHT
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Glutamate decarboxylase alpha Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Glutamate decarboxylase alpha Protein General Information | |
|---|---|
| Alternate Names | |
| ECK3502, gadS, b3517, JW3485 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | gadA |
| Entrez Gene ID | 948027 |
| RefSeq Protein Accession(s) | NP_417974.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P69908 |
| KEGG Gene ID(s) | ecj:JW3485 |
| General Description of Recombinant E. coli Glutamate decarboxylase alpha Protein. | |
| Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad syst helps to maintain a near-neutral intracellular pH when cells are exposed to extrely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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