PDF DatasheetE. coli Murein tetrapeptide carboxypeptidase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Murein tetrapeptide carboxypeptidase based upon sequence from: E. coli
Host: QP6956 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Murein tetrapeptide carboxypeptidase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6956.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Met304
Amino Acid Sequence: MSLFHLIAPS GYCIKQHAAL RGIQRLTDAG HQVNNVEVIA RRCERFAGTE TERLEDLNSL ARLTTPNTIV LAVRGGYGAS RLLADIDWQA LVARQQHDPL LICGHSDFTA IQCGLLAHGN VITFSGPMLV ANFGADELNA FTEHHFWLAL RNETFTIEWQ GEGPTCRAEG TLWGGNLAML ISLIGTPWMP KIENGILVLE DINEHPFRVE RMLLQLYHAG ILPRQKAIIL GSFSGSTPND YDAGYNLESV YAFLRSRLSI PLITGLDFGH EQRTVTLPLG AHAILNNTRE GTQLTISGHP VLKM
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Murein tetrapeptide carboxypeptidase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Murein tetrapeptide carboxypeptidase Protein General Information | |
|---|---|
| Alternate Names | |
| b1192, JW1181, ycgQ, ECK1180 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ldcA |
| Entrez Gene ID | 945756 |
| RefSeq Protein Accession(s) | NP_415710.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P76008 |
| KEGG Gene ID(s) | ecj:JW1181 |
| General Description of Recombinant E. coli Murein tetrapeptide carboxypeptidase Protein. | |
| Releases the terminal D-alanine residue from the Cytoplasmic domain tetrapeptide recycling product L-Ala-gamma-D-Glu-meso-Dap-D-Ala. To a lesser extent, can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc-tetrapeptide, GlcNAc-MurNAc-tetrapeptide, and GlcNAc-anhMurNAc-tetrapeptide. Does not act on murein sacculi or cross-linked muropeptides. The tripeptides produced by the LcdA reaction can then be reused as peptidoglycan building blocks; LcdA is thereby involved in murein recycling. Is also essential for viability during stationary phase. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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