PDF DatasheetE. coli Periplasmic serine endoprotease DegP Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Periplasmic serine endoprotease DegP based upon sequence from: E. coli
Host: QP7007 protein expressed in E.coli, Yeast..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Periplasmic serine endoprotease DegP, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7007.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala27 – Gln474
Amino Acid Sequence: AETSSATTAQ QMPSLAPMLE KVMPSVVSIN VEGSTTVNTP RMPRNFQQFF GDDSPFCQEG SPFQSSPFCQ GGQGGNGGGQ QQKFMALGSG VIIDADKGYV VTNNHVVDNA TVIKVQLSDG RKFDAKMVGK DPRSDIALIQ IQNPKNLTAI KMADSDALRV GDYTVAIGNP FGLGETVTSG IVSALGRSGL NAENYENFIQ TDAAINRGNS GGALVNLNGE LIGINTAILA PDGGNIGIGF AIPSNMVKNL TSQMVEYGQV KRGELGIMGT ELNSELAKAM KVDAQRGAFV SQVLPNSSAA KAGIKAGDVI TSLNGKPISS FAALRAQVGT MPVGSKLTLG LLRDGKQVNV NLELQQSSQN QVDSSSIFNG IEGAEMSNKG KDQGVVVNNV KTGTPAAQIG LKKGDVIIGA NQQAVKNIAE LRKVLDSKPS VLALNIQRGD STIYLLMQ
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Periplasmic serine endoprotease DegP Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Periplasmic serine endoprotease DegP Protein General Information | |
|---|---|
| Alternate Names | |
| ptd, ECK0160, JW0157, b0161, htrA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | degP |
| Entrez Gene ID | 947139 |
| RefSeq Protein Accession(s) | NP_414703.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0C0V0 |
| KEGG Gene ID(s) | ecj:JW0157 |
| General Description of Recombinant E. coli Periplasmic serine endoprotease DegP Protein. | |
| DegP acts as a chaperone at low tperatures but switches to a peptidase (heat shock protein) at higher tperatures. It degrades transiently denatured and unfolded proteins which accumulate in the periplasm following heat shock or other stress conditions. DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids. Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins. Its proteolytic activity is essential for the survival of cells at elevated tperatures. It can degrade IciA, ada, casein, globin and PapA. DegP shares specificity with DegQ. DegP is also involved in the biogenesis of partially folded outer-mbrane proteins (OMP). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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