PDF DatasheetC. botulinum Botulinum neurotoxin type A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Botulinum neurotoxin type A based upon sequence from: C. botulinum
Host: QP7051 protein expressed in E.coli, Yeast..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Clostridium botulinum Botulinum neurotoxin type A, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7051.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Met1 – Thr436
Amino Acid Sequence: MPFVNKQFNY KDPVNGVDIA YIKIPNVGQM QPVKAFKIHN KIWVIPERDT FTNPEEGDLN PPPEAKQVPV SYYDSTYLST DNEKDNYLKG VTKLFERIYS TDLGRMLLTS IVRGIPFWGG STIDTELKVI DTNCINVIQP DGSYRSEELN LVIIGPSADI IQFECKSFGH EVLNLTRNGY GSTQYIRFSP DFTFGFEESL EVDTNPLLGA GKFATDPAVT LAHELIHAGH RLYGIAINPN RVFKVNTNAY YEMSGLEVSF EELRTFGGHD AKFIDSLQEN EFRLYYYNKF KDIASTLNKA KSIVGTTASL QYMKNVFKEK YLLSEDTSGK FSVDKLKFDK LYKMLTEIYT EDNFVKFFKV LNRKTYLNFD KAVFKINIVP KVNYTIYDGF NLRNTNLAAN FNGQNTEINN MNFTKLKNFT GLFEFYKLLC VRGIIT
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of C. botulinum Botulinum neurotoxin type A Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant C. botulinum Botulinum neurotoxin type A Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | botA |
| RefSeq Protein Accession(s) | WP_003356619.1 |
| RefSeq mRNA Accession(s) | NZ_LFOS01000037.1, NZ_LHUL01000004.1 |
| UniProt ID(s) | P10845 |
| General Description of Recombinant C. botulinum Botulinum neurotoxin type A Protein. | |
| Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic mbrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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