PDF DatasheetRotavirus Rotavirus A Outer capsid protein VP4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Rotavirus A Outer capsid protein VP4 based upon sequence from: Rotavirus
Host: QP7052 protein expressed in E.coli, Yeast..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Rotavirus A (strain Human/United Kingdom/ST3/1975 G4-P2A[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain St. Thomas 3)) Rotavirus A Outer capsid protein VP4, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7052.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala247 – Tyr479
Amino Acid Sequence: AQVSEDIIIS KTSLWKEMQY NRDIIIRFKF NNSIIKLGGL GYKWSEISFK AANYQYNYLR DGEQVTAHTT CSVNGVNNFS YNGGLLPTHF SISRYEVIKE NSYVYVDYWD DSQAFRNMVY VRSLAANLNS VKCSGGNYNF QMPVGAWPVM SGGAVSLHFA GVTLSTQFTD FVSLNSLRFR FSLTVEEPPF SILRTRVSGL YGLPASNPNS GHEYYEIAGR FSLISLVPSN DDY
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Rotavirus Rotavirus A Outer capsid protein VP4 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Rotavirus Rotavirus A Outer capsid protein VP4 Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P11200 |
| General Description of Recombinant Rotavirus Rotavirus A Outer capsid protein VP4 Protein. | |
| Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. According to the considered strain, VP4 ses to essentially target sialic acid and/or the integrin heterodimer ITGA2/ITGB1 .Outer capsid protein VP5*: forms the spike “foot” and “body”. Acts as a mbrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment .VP8* forms the head of the spikes. It is the viral hagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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