PDF DatasheetRABV Nucleoprotein Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Nucleoprotein based upon sequence from: RABV
Host: QP7060 protein expressed in E.coli, Yeast..
Tag: His
Protein Construction: A DNA sequence encoding the Rabies virus (strain PM) (RABV) Nucleoprotein, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7060.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ser450
Amino Acid Sequence: MDADKIVFKV NNQVVSLKPE IIVDQYEYKY PAIKDLKKPC ITLGKAPDLN KAYKSVLSGM NAAKLDPDDV CSYLAAAMQF FEGTCPEDWT SYGILIARKG DRITPNSLVE IKRTDVEGNW ALTGGMELTR DPTVSEHASL VGLLLSLYRL SKISGQNTGN YKTNIADRIE QIFETAPFVK IVEHHTLMTT HKMCANWSTI PNFRFLAGTY DMFFSRIEHL YSAIRVGTVV TAYEDCSGLV SFTGFIKQIN LTAREAILYF FHKNFEEEIR RMFEPGQETA VPHSYFIHFR SLGLSGKSPY SSNAVGHVFN LIHFVGCYMG QVRSLNATVI AACAPHEMSV LGGYLGEEFF GKGTFERRFF RDEKELQEYE AAELTKTDVA LADDGTVNSD DEDYFSGETR SPEAVYTRIM MNGGRLKRSH IRRYVSVSSN HQARPNSFAE FLNKTYSNDS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of RABV Nucleoprotein Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant RABV Nucleoprotein Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | N |
| UniProt ID(s) | P15197 |
| General Description of Recombinant RABV Nucleoprotein Protein. | |
| Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it’s own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as tplate for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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