PDF DatasheetE. coli Oxygen-insensitive NADPH nitroreductase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Oxygen-insensitive NADPH nitroreductase based upon sequence from: E. coli
Host: QP7087 protein expressed in E.coli, Yeast..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Oxygen-insensitive NADPH nitroreductase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7087.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Arg240
Amino Acid Sequence: MTPTIELICG HRSIRHFTDE PISEAQREAI INSARATSSS SFLQCSSIIR ITDKALREEL VTLTGGQKHV AQAAEFWVFC ADFNRHLQIC PDAQLGLAEQ LLLGVVDTAM MAQNALIAAE SLGLGGVYIG GLRNNIEAVT KLLKLPQHVL PLFGLCLGWP ADNPDLKPRL PASILVHENS YQPLDKGALA QYDEQLAEYY LTRGSNNRRD TWSDHIRRTI IKESRPFILD YLHKQGWATR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Oxygen-insensitive NADPH nitroreductase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Oxygen-insensitive NADPH nitroreductase Protein General Information | |
|---|---|
| Alternate Names | |
| mdaA, ybjB, b0851, mda18, snrA, ECK0842, JW0835 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | nfsA |
| Entrez Gene ID | 945483 |
| RefSeq Protein Accession(s) | NP_415372.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P17117 |
| KEGG Gene ID(s) | ecj:JW0835 |
| General Description of Recombinant E. coli Oxygen-insensitive NADPH nitroreductase Protein. | |
| Reduction of nitroaromatic compounds using NADH. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major component of the oxygen-insensitive nitroreductase activity in E.coli. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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