PDF DatasheetInfluenza Influenza A H1N1 (strain A / Wilson-Smith / 1933) Nucleoprotein Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Influenza A H1N1 (strain A / Wilson-Smith / 1933) Nucleoprotein based upon sequence from: Influenza
Host: QP7106 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Influenza A virus (strain A/Wilson-Smith/1933 H1N1) Influenza A H1N1 (strain A/Wilson-Smith/1933) Nucleoprotein, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7106.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Asn498
Amino Acid Sequence: MATKGTKRSY EQMETDGERQ NATEIRASVG KMIGGIGRFY IQMCTELKLS DYEGRLIQNS LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYRRV DGKWMRELIL YDKEEIRRIW RQANNGDDAT AGLTHMMIWH SNLNDATYQR TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQRAMVD QVRESRNPGN AEFEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEREGYSLVG IDPFRLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT KVVPRGKLST RGVQIASNEN METMESSTLE LRSRYWAIRT RSGGNTNQQR ASSGQISIQP TFSVQRNLPF DRPTIMAAFT GNTEGRTSDM RTEIIRLMES ARPEDVSFQG RGVFELSDEK AASPIVPSFD MSNEGSYFFG DNAEEYDN
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Influenza Influenza A H1N1 (strain A / Wilson-Smith / 1933) Nucleoprotein Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Influenza Influenza A H1N1 (strain A / Wilson-Smith / 1933) Nucleoprotein Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NP |
| UniProt ID(s) | P15682 |
| General Description of Recombinant Influenza Influenza A H1N1 (strain A / Wilson-Smith / 1933) Nucleoprotein Protein. | |
| Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein’s nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein’s nuclear localization signals, targeting the RNP to the nucleus (By similarity). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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