PDF DatasheetE. coli ATP-dependent DNA helicase RecG Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ATP-dependent DNA helicase RecG based upon sequence from: E. coli
Host: QP7112 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) ATP-dependent DNA helicase RecG, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7112.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ala693
Amino Acid Sequence: MKGRLLDAVP LSSLTGVGAA LSNKLAKINL HTVQDLLLHL PLRYEDRTHL YPIGELLPGV YATVEGEVLN CNISFGGRRM MTCQISDGSG ILTMRFFNFS AAMKNSLAAG RRVLAYGEAK RGKYGAEMIH PEYRVQGDLS TPELQETLTP VYPTTEGVKQ ATLRKLTDQA LDLLDTCAIE ELLPPELSQG MMTLPEALRT LHRPPPTLQL SDLETGQHPA QRRLILEELL AHNLSMLALR AGAQRFHAQP LSANDTLKNK LLAALPFKPT GAQARVVAEI ERDMALDVPM MRLVQGDVGS GKTLVAALAA LRAIAHGKQV ALMAPTELLA EQHANNFRNW FAPLGIEVGW LAGKQKGKAR LAQQEAIASG QVQMIVGTHA IFQEQVQFNG LALVIIDEQH RFGVHQRLAL WEKGQQQGFH PHQLIMTATP IPRTLAMTAY ADLDTSVIDE LPPGRTPVTT VAIPDTRRTD IIDRVHHACI TEGRQAYWVC TLIEESELLE AQAAEATWEE LKLALPELNV GLVHGRMKPA EKQAVMASFK QGELHLLVAT TVIEVGVDVP NASLMIIENP ERLGLAQLHQ LRGRVGRGAV ASHCVLLYKT PLSKTAQIRL QVLRDSNDGF VIAQKDLEIR GPGELLGTRQ TGNAEFKVAD LLRDQAMIPE VQRLARHIHE RYPQQAKALI ERWMPETERY SNA
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli ATP-dependent DNA helicase RecG Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli ATP-dependent DNA helicase RecG Protein General Information | |
|---|---|
| Alternate Names | |
| radC, ECK3642, spoV, JW3627, b3652 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | recG |
| Entrez Gene ID | 948162 |
| RefSeq Protein Accession(s) | NP_418109.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P24230 |
| KEGG Gene ID(s) | ecj:JW3627 |
| General Description of Recombinant E. coli ATP-dependent DNA helicase RecG Protein. | |
| Critical role in recombination and DNA repair. Helpx process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3′- to 5′- polarity. Unwinds branched duplex DNA (Y-DNA). Has a role in constitutive stable DNA replication (CsdR) and R-loop formation. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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