PDF Datasheethepatitis Non-structural polyprotein pORF1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Non-structural polyprotein pORF1 based upon sequence from: hepatitis
Host: QP7125 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1) Non-structural polyprotein pORF1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7125.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Glu60 – Ile240
Amino Acid Sequence: EVFWNHPIQR VIHNELELYC RARSGRCLEI GAHPRSINDN PNVVHRCFLR PAGRDVQRWY TAPTRGPAAN CRRSALRGLP AADRTYCFDG FSGCNFPAET GIALYSLHDM SPSDVAEAMF RHGMTRLYAA LHLPPEVLLP PGTYRTASYL LIHDGRRVVV TYEGDTSAGY NHDVSNLRSW I
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of hepatitis Non-structural polyprotein pORF1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant hepatitis Non-structural polyprotein pORF1 Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ORF1 |
| UniProt ID(s) | P33424 |
| General Description of Recombinant hepatitis Non-structural polyprotein pORF1 Protein. | |
| Methyltransferase displays a Cytoplasmic domain capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction .RNA-directed RNA polymerase plays an essential role in the virus replication. Binds to the 3′-end of the genomic RNA, probably to initiate replication .. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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