PDF DatasheetE. coli Cytosine deaminase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Cytosine deaminase based upon sequence from E. coli
Host: QP7203 protein expressed in E.coli, Yeast..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Cytosine deaminase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7203.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ser2 – Arg427
Amino Acid Sequence: SNNALQTIIN ARLPGEEGLW QIHLQDGKIS AIDAQSGVMP ITENSLDAEQ GLVIPPFVEP HIHLDTTQTA GQPNWNQSGT LFEGIERWAE RKALLTHDDV KQRAWQTLKW QIANGIQHVR THVDVSDATL TALKAMLEVK QEVAPWIDLQ IVAFPQEGIL SYPNGEALLE EALRLGADVV GAIPHFEFTR EYGVESLHKT FALAQKYDRL IDVHCDEIDD EQSRFVETVA ALAHHEGMGA RVTASHTTAM HSYNGAYTSR LFRLLKMSGI NFVANPLVNI HLQGRFDTYP KRRGITRVKE MLESGINVCF GHDDVFDPWY PLGTANMLQV LHMGLHVCQL MGYGQINDGL NLITHHSART LNLQDYGIAA GNSANLIILP AENGFDALRR QVPVRYSVRG GKVIASTQPA QTTVYLEQPE AIDYKR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of E. coli Cytosine deaminase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Cytosine deaminase Protein General Information | |
|---|---|
| Alternate Names | |
| b0337, ECK0334, JW0328 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | codA |
| Entrez Gene ID | 944996 |
| RefSeq Protein Accession(s) | NP_414871.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P25524 |
| KEGG Gene ID(s) | ecj:JW0328 |
| General Description of Recombinant E. coli Cytosine deaminase Protein. | |
| Catalyzes the hydrolytic deamination of cytosine to uracil. Is involved in the pyrimidine salvage pathway, which allows the cell to utilize cytosine for pyrimidine nucleotide synthesis. Is also able to catalyze deamination of isoguanine, a mutagenic oxidation product of adenine in DNA, and of isocytosine. To a lesser extent, also catalyzes the conversion of 5-fluorocytosine (5FC) to 5-fluorouracil (5FU); this activity allows the formation of a cytotoxic chotherapeutic agent from a non-cytotoxic precursor. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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