PDF DatasheetE. coli RNA-splicing ligase RtcB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant RNA-splicing ligase RtcB based upon sequence from: E. coli
Host: QP7212 protein expressed in E.coli, Yeast..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) RNA-splicing ligase RtcB, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7212.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Gly408
Amino Acid Sequence: MNYELLTTEN APVKMWTKGV PVEADARQQL INTAKMPFIF KHIAVMPDVH LGKGSTIGSV IPTKGAIIPA AVGVDIGCGM NALRTALTAE DLPENLAELR QAIETAVPHG RTTGRCKRDK GAWENPPVNV DAKWAELEAG YQWLTQKYPR FLNTNNYKHL GTLGTGNHFI EICLDESDQV WIMLHSGSRG IGNAIGTYFI DLAQKEMQET LETLPSRDLA YFMEGTEYFD DYLKAVAWAQ LFASLNRDAM MENVVTALQS ITQKTVRQPQ TLAMEEINCH HNYVQKEQHF GEEIYVTRKG AVSARAGQYG IIPGSMGAKS FIVRGLGNEE SFCSCSHGAG RVMSRTKAKK LFSVEDQIRA TAHVECRKDA EVIDEIPMAY KDIDAVMAAQ SDLVEVIYTL RQVVCVKG
Purity: Greater than 81% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli RNA-splicing ligase RtcB Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli RNA-splicing ligase RtcB Protein General Information | |
|---|---|
| Alternate Names | |
| JW3384; b3421; ECK3407; yhgL | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | rtcB |
| Entrez Gene ID | 947929 |
| RefSeq Protein Accession(s) | NP_417879.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P46850 |
| KEGG Gene ID(s) | ecj:JW3384 |
| General Description of Recombinant E. coli RNA-splicing ligase RtcB Protein. | |
| RNA ligase that mediates the joining of broken tRNA-like stem-loop structures in case of tRNA damage. Probably participates to tRNA restriction-repair by ligating broken tRNA-like stem-loop structures with 2′,3′-cyclic phosphate and 5′-OH ends to form a splice junction with a 2′-OH, 3′,5′-phosphodiester, a step that requires GTP . Also acts as a DNA ligase in case of DNA damage by splicing ‘dirty’ DNA breaks, characterized by 3′-PO4 (or cyclic-PO4) and 5′-OH ends that cannot be sealed by classical DNA ligases | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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