PDF DatasheetM. tuberculosis Steroid C26-monooxygenase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Steroid C26-monooxygenase based upon sequence from: M. tuberculosis
Host: QP7253 protein expressed in E.coli, Yeast..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) Steroid C26-monooxygenase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7253.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – His433
Amino Acid Sequence: MSWNHQSVEI AVRRTTVPSP NLPPGFDFTD PAIYAERLPV AEFAELRSAA PIWWNGQDPG KGGGFHDGGF WAITKLNDVK EISRHSDVFS SYENGVIPRF KNDIAREDIE VQRFVMLNMD APHHTRLRKI ISRGFTPRAV GRLHDELQER AQKIAAEAAA AGSGDFVEQV SCELPLQAIA GLLGVPQEDR GKLFHWSNEM TGNEDPEYAH IDPKASSAEL IGYAMKMAEE KAKNPADDIV TQLIQADIDG EKLSDDEFGF FVVMLAVAGN ETTRNSITQG MMAFAEHPDQ WELYKKVRPE TAADEIVRWA TPVTAFQRTA LRDYELSGVQ IKKGQRVVMF YRSANFDEEV FQDPFTFNIL RNPNPHVGFG GTGAHYCIGA NLARMTINLI FNAVADHMPD LKPISAPERL RSGWLNGIKH WQVDYTGRCP VAH
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of M. tuberculosis Steroid C26-monooxygenase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant M. tuberculosis Steroid C26-monooxygenase Protein General Information | |
|---|---|
| Alternate Names | |
| Rv3545c | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | cyp125 |
| Entrez Gene ID | 887782 |
| RefSeq Protein Accession(s) | NP_218062.1 |
| RefSeq mRNA Accession(s) | NC_000962.3, NZ_KK339374.1 |
| UniProt ID(s) | P9WPP1 |
| KEGG Gene ID(s) | mtu:Rv3545c |
| General Description of Recombinant M. tuberculosis Steroid C26-monooxygenase Protein. | |
| Catalyzes the C-27 hydroxylation of cholest-4-en-3-one and cholesterol and subsequently oxidizes the alcohol of the former to the cholest-4-en-3-one-27-oic acid via the aldehyde intermediate. Not required to incorporate the cholesterol side-chain carbon atoms into cellular lipids. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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