PDF DatasheetE. coli Beta-lactamase TEM Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Beta-lactamase TEM based upon sequence from: E. coli
Host: QP7256 protein expressed in E.coli, Yeast..
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Beta-lactamase TEM, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7256.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: His24 – Trp286
Amino Acid Sequence: HPETLVKVKD AEDQLGARVG YIELDLNSGK ILESFRPEER FPMMSTFKVL LCGAVLSRVD AGQEQLGRRI HYSQNDLVEY SPVTEKHLTD GMTVRELCSA AITMSDNTAA NLLLTTIGGP KELTAFLHNM GDHVTRLDRW EPELNEAIPN DERDTTMPAA MATTLRKLLT GELLTLASRQ QLIDWMEADK VAGPLLRSAL PAGWFIADKS GAGERGSRGI IAALGPDGKP SRIVVIYTTG SQATMDERNR QIAEIGASLI KHW
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Beta-lactamase TEM Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Beta-lactamase TEM Protein General Information | |
|---|---|
| Alternate Names | |
| p3521_p083; D616_p87011; D616_p141023; pIGAL1_03; pEK516_p24; D616_p89001; D616_p118028; pECL8_p095; p3521_p066; NDM1Dok01_N0178; D616_p59037; pHN3A11_016; D616_p107018; D616_p156055; O2R_124; HUS41_pI0062; D616_p121056; D616_p139124; pOLA52_03; D616_p119009; ETN48_p0069; D616_p58029; pHN7A8_020; D616_p109104; D616_p137091; D616_p148018; D616_p149004; D616_p1002; D616_p127015; IPF_160; rpec180_8; D616_p78029; D616_p108018; pC15-1a_020; D616_p80068; pLEW517_p68; pEK204_p11; pECBactecp30 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | bla |
| Entrez Gene ID | 10076131 |
| RefSeq Protein Accession(s) | NP_943295.1 |
| RefSeq mRNA Accession(s) | NC_005248.1, NC_005327.1, NZ_NIZE01000137.1, NG_050277.1, NC_009132.1, NC_010378.1, NC_010558.1, NC_013120.1, NC_013121.1, NC_014383.1, NC_014384.1, NC_014615.1, NC_014843.1, NC_019047.1, NC_018994.1, NC_018995.1, NC_019056.1, NC_019062.1, NC_019063.1, NC_019073.1, NC_019088.1, NC_019091.1, NC_019095.1, NC_020278.2, NC_022992.1, NC_022996.1, NC_023277.2, NC_024960.1, NC_024961.1, NC_024967.1, NC_024977.1, NC_025106.1, NC_025139.1, NC_025141.1, NC_025167.1, NC_025175.1, NC_025183.1, NC_006671.1 |
| UniProt ID(s) | P62593 |
| KEGG Gene ID(s) | ag:AAB59737 |
| General Description of Recombinant E. coli Beta-lactamase TEM Protein. | |
| T-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. T-3 and T-4 are capable of hydrolyzing cefotaxime and ceftazidime. T-5 is capable of hydrolyzing ceftazidime. T-6 is capable of hydrolyzing ceftazidime and aztreonam. T-8/CAZ-2, T-16/CAZ-7 and T-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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