PDF DatasheetE. coli ruvA Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ruvA based upon sequence from: E. coli
Host: QP7321 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) ruvA, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7321.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Leu203
Amino Acid Sequence: MIGRLRGIII EKQPPLVLIE VGGVGYEVHM PMTCFYELPE AGQEAIVFTH FVVREDAQLL YGFNNKQERT LFKELIKTNG VGPKLALAIL SGMSAQQFVN AVEREEVGAL VKLPGIGKKT AERLIVEMKD RFKGLHGDLF TPAADLVLTS PASPATDDAE QEAVAALVAL GYKPQEASRM VSKIARPDAS SETLIREALR AAL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli ruvA Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli ruvA Protein General Information | |
|---|---|
| Alternate Names | |
| ECK1862; JW1850; b1861; Holliday junction ATP-dependent DNA helicase Ru | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ruvA |
| Entrez Gene ID | 946369 |
| RefSeq Protein Accession(s) | NP_416375.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0A809 |
| KEGG Gene ID(s) | ecj:JW1850 |
| General Description of Recombinant E. coli ruvA Protein. | |
| The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.