PDF DatasheetE. coli KHG / KDPG aldolase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant KHG / KDPG aldolase based upon sequence from: E. coli
Host: QP7323 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) KHG/KDPG aldolase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7323.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Leu213
Amino Acid Sequence: MKNWKTSAES ILTTGPVVPV IVVKKLEHAV PMAKALVAGG VRVLEVTLRT ECAVDAIRAI AKEVPEAIVG AGTVLNPQQL AEVTEAGAQF AISPGLTEPL LKAATEGTIP LIPGISTVSE LMLGMDYGLK EFKFFPAEAN GGVKALQAIA GPFSQVRFCP TGGISPANYR DYLALKSVLC IGGSWLVPAD ALEAGDYDRI TKLAREAVEG AKL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli KHG / KDPG aldolase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli KHG / KDPG aldolase Protein General Information | |
|---|---|
| Alternate Names | |
| ECK1851; JW1839; hga; b1850; kga; kdgA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | eda |
| Entrez Gene ID | 946367 |
| RefSeq Protein Accession(s) | NP_416364.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0A955 |
| COSMIC ID Link(s) | eda |
| KEGG Gene ID(s) | ecj:JW1839 |
| General Description of Recombinant E. coli KHG / KDPG aldolase Protein. | |
| Involved in the degradation of glucose via the Entner-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-Keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with si-facial selectivity. It accepts some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate, and fluorobutanoate. It has a preference for the S-configuration at C2 of the electrophile. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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