PDF DatasheetE. coli Methionine aminopeptidase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Methionine aminopeptidase based upon sequence from: E. coli
Host: QP7333 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Methionine aminopeptidase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7333.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Glu264
Amino Acid Sequence: AISIKTPEDI EKMRVAGRLA AEVLEMIEPY VKPGVSTGEL DRICNDYIVN EQHAVSACLG YHGYPKSVCI SINEVVCHGI PDDAKLLKDG DIVNIDVTVI KDGFHGDTSK MFIVGKPTIM GERLCRITQE SLYLALRMVK PGINLREIGA AIQKFVEAEG FSVVREYCGH GIGRGFHEEP QVLHYDSRET NVVLKPGMTF TIEPMVNAGK KEIRTMKDGW TVKTKDRSLS AQYEHTIVVT DNGCEILTLR KDDTIPAIIS HDE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Methionine aminopeptidase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Methionine aminopeptidase Protein General Information | |
|---|---|
| Alternate Names | |
| ECK0166; JW0163; pepM; b0168 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | map |
| Entrez Gene ID | 947882 |
| RefSeq Protein Accession(s) | NP_414710.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0AE18 |
| KEGG Gene ID(s) | ecj:JW0163 |
| General Description of Recombinant E. coli Methionine aminopeptidase Protein. | |
| Roves the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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