PDF DatasheetHuman HPV B19 Capsid protein VP1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HPV B19 Capsid protein VP1 based upon sequence from: Human
Host: QP7371 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) HPV B19 Capsid protein VP1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7371.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met228 – Leu781
Amino Acid Sequence: MTSVNSAEAS TGAGGGGSNS VKSMWSEGAT FSANSVTCTF SRQFLIPYDP EHHYKVFSPA ASSCHNASGK EAKVCTISPI MGYSTPWRYL DFNALNLFFS PLEFQHLIEN YGSIAPDALT VTISEIAVKD VTDKTGGGVQ VTDSTTGRLC MLVDHEYKYP YVLGQGQDTL APELPIWVYF PPQYAYLTVG DVNTQGISGD SKKLASEESA FYVLEHSSFQ LLGTGGTASM SYKFPPVPPE NLEGCSQHFY EMYNPLYGSR LGVPDTLGGD PKFRSLTHED HAIQPQNFMP GPLVNSVSTK EGDSSNTGAG KALTGLSTGT SQNTRISLRP GPVSQPYHHW DTDKYVTGIN AISHGQTTYG NAEDKEYQQG VGRFPNEKEQ LKQLQGLNMH TYFPNKGTQQ YTDQIERPLM VGSVWNRRAL HYESQLWSKI PNLDDSFKTQ FAALGGWGLH QPPPQIFLKI LPQSGPIGGI KSMGITTLVQ YAVGIMTVTM TFKLGPRKAT GRWNPQPGVY PPHAAGHLPY VLYDPTATDA KQHHRHGYEK PEELWTAKSR VHPL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human HPV B19 Capsid protein VP1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human HPV B19 Capsid protein VP1 Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P07299 |
| General Description of Recombinant Human HPV B19 Capsid protein VP1 Protein. | |
| Capsid protein self-assbles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors, such as the glycosphingolipid globoside or the integrin heterodimer ITGAV/ITGB1. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangents leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal mbrane during entry into host cell. IntraCytoplasmic domain transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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