PDF DatasheetE. coli LexA repressor Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant LexA repressor based upon sequence from: E. coli
Host: QP7390 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) LexA repressor, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7390.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Leu202
Amino Acid Sequence: MKALTARQQE VFDLIRDHIS QTGMPPTRAE IAQRLGFRSP NAAEEHLKAL ARKGVIEIVS GASRGIRLLQ EEEEGLPLVG RVAAGEPLLA QQHIEGHYQV DPSLFKPNAD FLLRVSGMSM KDIGIMDGDL LAVHKTQDVR NGQVVVARID DEVTVKRLKK QGNKVELLPE NSEFKPIVVD LRQQSFTIEG LAVGVIRNGD WL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli LexA repressor Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli LexA repressor Protein General Information | |
|---|---|
| Alternate Names | |
| tsl; umuA; b4043; ECK4035; JW4003; spr; exrA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | lexA |
| Entrez Gene ID | 948544 |
| RefSeq Protein Accession(s) | NP_418467.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0A7C2 |
| KEGG Gene ID(s) | ecj:JW4003 |
| General Description of Recombinant E. coli LexA repressor Protein. | |
| Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5′-CTGTATATATATACAG-3′. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment .The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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