PDF DatasheetE. coli ruvB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ruvB based upon sequence from: E. coli
Host: QP7392 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) ruvB, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7392.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Pro336
Amino Acid Sequence: MIEADRLISA GTTLPEDVAD RAIRPKLLEE YVGQPQVRSQ MEIFIKAAKL RGDALDHLLI FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM LTNLEPHDVL FIDEIHRLSP VVEEVLYPAM EDYQLDIMIG EGPAARSIKI DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR LEFYQVPDLQ YIVSRSARFM GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG TISADIAAQA LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV LEPYLIQQGF LQRTPRGRMA TTRAWNHFGI TPPEMP
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli ruvB Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli ruvB Protein General Information | |
|---|---|
| Alternate Names | |
| ECK1861; JW1849; b1860; Holliday junction ATP-dependent DNA helicase Ru | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ruvB |
| Entrez Gene ID | 946371 |
| RefSeq Protein Accession(s) | NP_416374.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0A812 |
| KEGG Gene ID(s) | ecj:JW1849 |
| General Description of Recombinant E. coli ruvB Protein. | |
| The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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