PDF DatasheetE. coli ruvC Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ruvC based upon sequence from: E. coli
Host: QP7393 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) ruvC, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7393.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Arg173
Amino Acid Sequence: AIILGIDPGS RVTGYGVIRQ VGRQLSYLGS GCIRTKVDDL PSRLKLIYAG VTEIITQFQP DYFAIEQVFM AKNADSALKL GQARGVAIVA AVNQELPVFE YAARQVKQTV VGIGSAEKSQ VQHMVRTLLK LPANPQADAA DALAIAITHC HVSQNAMQMS ESRLNLARGR LR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli ruvC Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli ruvC Protein General Information | |
|---|---|
| Alternate Names | |
| JW1852; b1863; ECK1864; Crossover junction endodeoxyribonuclease Ru | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ruvC |
| Entrez Gene ID | 946378 |
| RefSeq Protein Accession(s) | NP_416377.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0A814 |
| KEGG Gene ID(s) | ecj:JW1852 |
| General Description of Recombinant E. coli ruvC Protein. | |
| Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5′-terminal phosphate and a 3′-terminal hydroxyl group. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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