PDF DatasheetHuman Protein E6 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Protein E6 based on published sequence from: Human
Host: QP7409 protein expressed in E.coli, Yeast.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Protein E6, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7409.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Leu158
Amino Acid Sequence: MHQKRTAMFQ DPQERPRKLP QLCTELQTTI HDIILECVYC KQQLLRREVY DFAFRDLCIV YRDGNPYAVC DKCLKFYSKI SEYRHYCYSL YGTTLEQQYN KPLCDLLIRC INCQKPLCPE EKQRHLDKKQ RFHNIRGRWT GRCMSCCRSS RTRRETQL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Protein E6 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Protein E6 Protein General Information | |
|---|---|
| Alternate Names | |
| HpV16gp1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | E6 |
| Entrez Gene ID | 1489078 |
| RefSeq Protein Accession(s) | NP_041325.1 |
| RefSeq mRNA Accession(s) | NC_001526.4 |
| UniProt ID(s) | P03126 |
| KEGG Gene ID(s) | vg:1489078 |
| General Description of Recombinant Human Protein E6 Protein. | |
| Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting th to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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