PDF DatasheetE. coli Modification methylase EcoRV Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Modification methylase EcoRV based upon sequence from: E. coli
Host: QP7415 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Modification methylase EcoRV, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7415.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Glu298
Amino Acid Sequence: MKDKVFVPPI KSQGIKTKLV PCIKRIVPKN FNGVWVEPFM GTGVVAFNVA PKDALLCDTN PHLISFYNAL KNKDITGDLV KDFLYREGEK LLLSNGEYYY EVRERFNNYK EPLDFLFLNR SCFNGMIRFN SKGGFNVPFC KKPNRFAQAY ITKISNQVDR ISEIISKGNY TFLCQSFEKT IGMVNRDDVV YCDPPYIGRH VDYFNSWGER DERLLFETLS SLNATFITST WHHNDYRENK YVRDLWSSFR ILTKEHFYHV GASEKNRSPM VEALITNIAK DIIDHIEKSS GDILVIEE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Modification methylase EcoRV Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Modification methylase EcoRV Protein General Information | |
|---|---|
| Alternate Names | |
| pLG13_07 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ecoRVM |
| Entrez Gene ID | 1446622 |
| RefSeq Protein Accession(s) | NP_863581.1 |
| RefSeq mRNA Accession(s) | NC_005019.1 |
| UniProt ID(s) | P04393 |
| General Description of Recombinant E. coli Modification methylase EcoRV Protein. | |
| This methylase recognizes the double-stranded sequence GATATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the EcoRV endonuclease. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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