PDF DatasheetC. botulinum clpP Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant clpP based upon sequence from: C. botulinum
Host: QP7429 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) clpP, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7429.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Lys194
Amino Acid Sequence: MSLVPVVVEQ TNRGERSYDI YSRLLKDRII MLSEEVNDTT ASLIVAQLLF LEAEDPDKDI HLYINSPGGS ITSGMAIYDT MQYIKPDVST ICVGMAASMG AFLLAAGAKG KRYALPNSEV MIHQPLGGFR GQATDIGIHA ERILKMKKKL NTILSDRTGK PLEQVELDTE RDHFLSAEEA KEYGLIDEVI DKKK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of C. botulinum clpP Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant C. botulinum clpP Protein General Information | |
|---|---|
| Alternate Names | |
| CLC_3142; CBO3231; ATP-dependent Clp protease proteolytic subun | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | clpP |
| Entrez Gene ID | 5187372 |
| RefSeq Protein Accession(s) | WP_003357557.1 |
| RefSeq mRNA Accession(s) | NC_009698.1, NC_009495.1 |
| UniProt ID(s) | A5I6W1 |
| COSMIC ID Link(s) | clpP |
| KEGG Gene ID(s) | cbh:CLC_3142 |
| General Description of Recombinant C. botulinum clpP Protein. | |
| Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). |
|
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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