PDF DatasheetRotavirus Non-structural glycoprotein 4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Non-structural glycoprotein 4 based upon sequence from: Rotavirus
Host: QP7439 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Rotavirus A (isolate Monkey/South Africa/SA11-H96/1958 G3-P5B[2]-I2-R2-C5-M5-A5-N5-T5-E2-H5) (RV-A) (Simian Agent 11 (isolate SI/South Africa/H96/58)) Non-structural glycoprotein 4, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7439.
Bioactivity Data: Untested
Full Length? Cytoplasmic Domain
Expression Region: Pro52 – Met175
Amino Acid Sequence: PTMKIALKTS KCSYKVVKYC IVTIFNTLLK LAGYKEQITT KDEIEKQMDR VVKEMRRQLE MIDKLTTREI EQVELLKRIY DKLTVQTTGE IDMTKEINQK NVRTLEEWES GKNPYEPREV TAAM
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Rotavirus Non-structural glycoprotein 4 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Rotavirus Non-structural glycoprotein 4 Protein General Information | |
|---|---|
| Alternate Names | |
| RVA_s10_gp1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NSP4 |
| Entrez Gene ID | 7011361 |
| RefSeq Protein Accession(s) | YP_002302223.1 |
| RefSeq mRNA Accession(s) | NC_011504.2 |
| UniProt ID(s) | A2T3Q0 |
| KEGG Gene ID(s) | vg:7011361 |
| General Description of Recombinant Rotavirus Non-structural glycoprotein 4 Protein. | |
| Involved in virus morphogenesis. Functions as a receptor for the immature double-layered inner capsid particle (ICP) which transiently buds into the lumen of the rough endoplasmic reticulum during viral maturation .Enterotoxin that causes a phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Increased concentration of intracellular calcium disrupts the cytoskeleton and the tight junctions, raising the paracellular permeability. Potentiates chloride ion secretion through a calcium ion-dependent signaling pathway, inducing age-dependent diarrhea. To perform this enterotoxigenic role in vivo, NSP4 is probably released from infected enterocytes in a soluble form capable of diffusing within the intestinal lumen and interacting with the plasma mbrane receptors on neighboring epithelial cells. Possible receptors for NSP4 are alpha-1/beta-1 and alpha-2/beta-1 integrin heterodimers . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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