PDF DatasheetHuman Histone-lysine N-methyltransferase SETDB1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Histone-lysine N-methyltransferase SETDB1 based upon sequence from: Human
Isoform: Isoform 2
Host: QP7573 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Histone-lysine N-methyltransferase SETDB1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7573.
Bioactivity Data: Untested
Full Length? Full Length of Isoform 2
Expression Region: Met1 – Thr397
Amino Acid Sequence: MSSLPGCIGL DAATATVESE EIAELQQAVV EELGISMEEL RHFIDEELEK MDCVQQRKKQ LAELETWVIQ KESEVAHVDQ LFDDASRAVT NCESLVKDFY SKLGLQYRDS SSEDESSRPT EIIEIPDEDD DVLSIDSGDA GSRTPKDQKL REAMAALRKS AQDVQKFMDA VNKKSSSQDL HKGTLSQMSG ELSKDGDLIV SMRILGKKRT KTWHKGTLIA IQTVGPGKKY KVKFDNKGKS LLSGNHIAYD YHPPADKLYV GSRVVAKYKD GNQVWLYAGI VAETPNVKNK LRFLIFFDDG YASYVTQSEL YPICRPLKKT WEDIEDISCR DFIEEYVTAY PNRPMVLLKS GQLIKTEWEG TWWKSRVEEV DGSLVRILFL VLFFSTILEA EVGGGGT
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Histone-lysine N-methyltransferase SETDB1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Histone-lysine N-methyltransferase SETDB1 Protein General Information | |
|---|---|
| Alternate Names | |
| ESET; KMT1E; H3-K9-HMTase4; KG1T; TDRD21 | |
| Molecular Weight | |
| Isoform 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SETDB1 |
| Entrez Gene ID | 9869 |
| Ensemble Gene ID | ENSG00000143379 |
| RefSeq Protein Accession(s) | NP_001138887.1 |
| RefSeq mRNA Accession(s) | NM_001145415.1, NM_001243491.1, NM_012432.3, XM_017002955.1 |
| UniProt ID(s) | Q15047 |
| UniGene ID(s) | Hs.643565 |
| HGNC ID(s) | HGNC:10761 |
| COSMIC ID Link(s) | SETDB1 |
| KEGG Gene ID(s) | hsa:9869 |
| PharmGKB ID(s) | PA35679 |
| General Description of Recombinant Human Histone-lysine N-methyltransferase SETDB1 Protein. | |
| Histone methyltransferase that specifically trimethylates ‘Lys-9’ of histone H3. H3 ‘Lys-9’ trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 ‘Lys-9’ trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone ‘Lys-9′ trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing . The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3’-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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