PDF DatasheetHuman MED1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MED1 based upon sequence from: Human
Host: QP7584 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) MED1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7584.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Phe878 – Phe1031
Amino Acid Sequence: FGEEYFDESS QSGDNDDFKG FASQALNTLG VPMLGGDNGE TKFKGNNQAD TVDFSIISVA GKALAPADLM EHHSGSQGPL LTTGDLGKEK TQKRVKEGNG TSNSTLSGPG LDSKPGKRSR TPSNDGKSKD KPPKRKKADT EGKSPSHSSS NRPF
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human MED1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human MED1 Protein General Information | |
|---|---|
| Alternate Names | |
| DRIP205; PBP; RB18A; CRSP1; CRSP200; PPARGBP; DRIP230; TRAP220; PPARBP; TRIP2; Mediator of RNA polymerase II transcription subunit | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MED1 |
| Entrez Gene ID | 5469 |
| Ensemble Gene ID | ENSG00000125686 |
| RefSeq Protein Accession(s) | NP_004765.2 |
| RefSeq mRNA Accession(s) | NM_004774.3 |
| UniProt ID(s) | Q15648 |
| UniGene ID(s) | Hs.643754 |
| HGNC ID(s) | HGNC:9234 |
| COSMIC ID Link(s) | MED1 |
| KEGG Gene ID(s) | hsa:5469 |
| PharmGKB ID(s) | PA33556 |
| General Description of Recombinant Human MED1 Protein. | |
| Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.