PDF DatasheetHuman Angiopoietin 1 / ANG1 / ANGPT1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Angiopoietin 1 / ANG1 / ANGPT1 based upon sequence from: Human
Host: QP7600 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Angiopoietin 1 / ANG1 / ANGPT1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7600.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: His16 – Phe498
Amino Acid Sequence: HIGCSNQRRS PENSGRRYNR IQHGQCAYTF ILPEHDGNCR ESTTDQYNTN ALQRDAPHVE PDFSSQKLQH LEHVMENYTQ WLQKLENYIV ENMKSEMAQI QQNAVQNHTA TMLEIGTSLL SQTAEQTRKL TDVETQVLNQ TSRLEIQLLE NSLSTYKLEK QLLQQTNEIL KIHEKNSLLE HKILEMEGKH KEELDTLKEE KENLQGLVTR QTYIIQELEK QLNRATTNNS VLQKQQLELM DTVHNLVNLC TKEGVLLKGG KREEEKPFRD CADVYQAGFN KSGIYTIYIN NMPEPKKVFC NMDVNGGGWT VIQHREDGSL DFQRGWKEYK MGFGNPSGEY WLGNEFIFAI TSQRQYMLRI ELMDWEGNRA YSQYDRFHIG NEKQNYRLYL KGHTGTAGKQ SSLILHGADF STKDADNDNC MCKCALMLTG GWWFDACGPS NLNGMFYTAG QNHGKLNGIK WHYFKGPSYS LRSTTMMIRP LDF
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Angiopoietin 1 / ANG1 / ANGPT1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Angiopoietin 1 / ANG1 / ANGPT1 Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Angpt1 |
| Entrez Gene ID | 284 |
| Ensemble Gene ID | ENSG00000154188 |
| RefSeq Protein Accession(s) | NP_001137.2 |
| RefSeq mRNA Accession(s) | NM_001146.4, NM_001199859.2, NM_001314051.1 |
| UniProt ID(s) | Q15389 |
| UniGene ID(s) | Hs.369675 |
| HGNC ID(s) | HGNC:484 |
| COSMIC ID Link(s) | Angpt1 |
| KEGG Gene ID(s) | hsa:284 |
| PharmGKB ID(s) | PA24791 |
| General Description of Recombinant Human Angiopoietin 1 / ANG1 / ANGPT1 Protein. | |
| Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during bryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the Extracellular domain matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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